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Segments of 85–90 nucleotides of PepYVMLV DNA-A and DNA-B were amplified by PCR using the GoTaq Polymerase kit (Promega) with specific primers PepYVMLV-A-F/PepYVMLV-A-R and PepYVMLV-B-F/PepYVMLV-B-R according to Ouattara et al. (2020)15. PCR products were then migrated in a 1% agarose gel and purified using NucleoSpin® Gel and PCR Clean-up (Macherey Nagel). The resulting amplicons were then used as templates to produce segment-specific probes using the Invitrogen and Alexa Fluor Bioprime DNA Labelling Kit (Alexa Fluor 488 and 568) as described elsewhere41.
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