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Liquid handling and cassettes

SK Simon Klingler
JH Jason P. Holland
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All components for the disposable cassette system were either custom-manufactured or purchased from commercial vendors (B. Braun Melsungen AG, Meslungen, Germany, or BD, Heidelberg, Germany). The cassettes use flexible, high chemical resistance Tygon tubing. For purification, custom-made SK-10 separation columns (Econo-Pac chromatography columns, Bio-Rad Laboratories, Hercules, CA, USA) filled with 8.3 mL of pre-soaked Sephadex® G-100 (Merck, Darmstadt, Germany) were constructed. The stationary phase of the SK-10 columns was capped with filter-frits and columns were eluted with sterile PBS (pH7.4).

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