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Analysis of vector genome integrity
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To analyze the genome integrity of scAAV vectors, 5 × 1011 AAV particles (vector genomes as quantified by qPCR) were treated with Proteinase K (QIAGEN) for 30 min at 56°C. Next, vector DNA was extracted using the DNeasy Blood & Tissue Kit (QIAGEN), mixed with 6× purple gel loading dye (NEB), and loaded next to the 1 Kb Plus DNA Ladder (Thermo Fisher) onto a native 1% agarose gel stained with ethidium bromide (Roth, Karlsruhe, Germany). Electrophoresis was performed in 1× TAE (tris-acetate-EDTA) buffer at 120 V for 30 min.
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