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Lactate production and glucose consumption were assessed with a lactate assay kit (Solarbio) or glucose assay kit (Solarbio) according to the manufacturer's instructions. The ECAR and OCR of transfected NSCLC cells were evaluated by using an XFe 96 Extracellular Flux analyzer (Seahorse Bioscience) with an XF glycolysis stress test Kit (Seahorse Bioscience) or XF Cell Mito Stress Test Kit (Seahorse Bioscience) according to the manufacturer's instructions.
Copyright and License information: ©2021 The Authors. published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
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