2.3. Cell Culture and Intervention

BV2 cells were cultivated in DMEM supplemented with 10% fetal bovine serum and 1% penicillin and streptomycin mixture in a humidified incubator with 5% CO₂ at 37°C. BV2 cells were arranged to control, lipopolysaccharide (LPS), LPS+sesamol, and compound C (LPS+sesamol+compound C) groups. Cells were incubated with 10 μM sesamol dissolved in PBS for 2 h and then suffered from LPS (1 μg/mL) for 24 h to stimulate inflammation. To explore whether the AMPK pathway participates in anti-inflammation of sesamol, cells were pretreated with compound C (a specific AMPK inhibitor) for 2 h before sesamol treatment.