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Static model digesta ultrafiltration

AH Alexandra E. Hall
CM Carmen I. Moraru
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Prior to the BCA, OPA, and glucose assays, SG and SI were defrosted and underwent serial ultrafiltration in Amicon® Ultra-4 centrifugal filter devices (MilliporeSigma, Burlington, MA, USA) with molecular weight cutoffs of 50 kDa, 30 kDa, and 10 kDa. Ultrafiltration occurred via centrifugation of digesta at 7500 × g, followed by a water rinse centrifugation step for each molecular weight cutoff centrifugal filter device. Ultimately, the SG and SI filtrate consisted of molecules less than 10 kDa. Therefore, all analyses, with the exception of SDS-PAGE, for both the dynamic and static model were performed on filtrate less than 10 kDa.

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