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Enzyme linked immunospot (ELISPOT) assay

TJ Tanner M. Johanns
JW Jeffrey P. Ward
CM Christopher A. Miller
CW Courtney Wilson
DK Dale K. Kobayashi
DB Diane Bender
YF Yujie Fu
AA Anton Alexandrov
EM Elaine R. Mardis
MA Maxim N. Artyomov
RS Robert D. Schreiber
GD Gavin P. Dunn
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Naïve splenocytes were plated at a concentration of 100,000–200,000 cells/well in 100 µL serum-free C.T.L. media (Cellular Technology, Ltd., Shaker Heights, OH) on precoated murine IFNγ ELISPOT plates (Cellular Technology, Ltd., Shaker Heights, OH). Harvested TIL were added at a final concentration of 25,000 cells/well in 200 µL with peptide (10 µM) (Peptide 2.0 Inc., Chantilly, VA). Concavalin A (1 µg/well) was a positive control. Plates were incubated overnight at 37°C and analyzed using the C.T.L. ImmunoSpot kit (Cellular Technology, Ltd., Shaker Heights, OH).

Copyright and License information:  ©2016

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