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The OS cells were seeded in 6-well plates and transfected with Cdc20 cDNA or shRNA and empty vector. After cells almost covered the well, absorbed the supernatant and scratched the cell with a yellow pipette tip. Subsequently, the cells were washed with PBS, and then the scratched zone was taken photos with the microscope at 0 h, 10 h and 20 h, respectively.
Copyright and License information: ©2017 Taylor & Francis
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