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Clonogenic cell survival assay

WY Wei Yang
HY Hongquan Yu
YS Yueming Shen
YL Yingying Liu
ZY Zhanshan Yang
TS Ting Sun
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Cells were irradiated with X-ray (6 MV, the dose rate was 200 cGy/min) by a PRIMUS accelerator (SIEMENS Medical Solutions, Erlangen, Germany) at room temperature. After irradiation, a specific number of cells (100 for cells irradiated with 0 or 2 Gy, 200 for 4 Gy and 2000 for 6, 8 and 10 Gy) was plated in petri dishes in triplicate for clonogenic assay. Then the cells were incubated for 12 days. Colonies were fixed by 37% formaldehyde solution and stained with crystal violet and colonies of more than 50 cells were counted. Furthermore, the cell survival fraction was counted out and the mean lethal dose (D0) was calculated by the linear quadratic model.

Copyright and License information:  ©2016 Yang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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