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For TUNEL (Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) assays, larvae were incubated in 0.1 M glycine/PBS solution for 10 min and then rinsed with PBT-2 three times for 10 minutes each. The larvae were then processed using the In Situ Cell Death Detection Kit (Roche, Nutlet, NJ, USA; cat. no.11684795910) following the directions supplied by the manufacturer.
Copyright and License information: ©2016 He et al.
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