The intervention group

YC Yan Cao
XY Xuan Yin
FS Francisca Soto-Aguilar
YL Yiping Liu
PY Ping Yin
JW Junyi Wu
BZ Bochang Zhu
WL Wentao Li
LL Lixing Lao
SX Shifen Xu
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The intervention group will be treated with the real tube-needling method, while lying in the supine position and wearing an eye-patch. After routine skin sterilizing, DU20, DU24, and EX-HN3 will be punctured obliquely 10 mm deep into the skin; EX-HN22 and SP6 will be punctured 15 mm deep in a direction perpendicular to the skin, and HT7 will be punctured perpendicularly to a depth of 5 mm. Licensed and experienced acupuncturists will conduct needle manipulation including lifting, thrusting, and rotating to obtain the proper needling sensation (De-qi sensation). Then they will stick a 2 × 2-cm piece of tape on the skin beside each needle. After a 20-min retention period, the needles and tapes will be removed simultaneously.

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