Five NSCLC cancer cell lines (A549, SPC-A1, H1299, 95D, SK-MES-1, and NCI-H520) and human bronchial epithelial (16HBE) cell were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China). Cells were maintained in RPMI 1640 (GIBCO-BRL; Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS) and antibiotics and cultured at 37°C in humidified air with 5% CO2.
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