2.4. Assessment of Whole-Body Glucose Tolerance

At PND60 and PND120, intraperitoneal glucose tolerance tests (IPGTTs) were performed to assess whole-body glucose tolerance. Following an overnight (16 h) fast, baseline glucose was measured and a bolus injection of 50% dextrose (1 g/kg body weight) was immediately administered intraperitoneally. Blood was sampled at the peripheral ear vein every 10 min during the 20 min post-injection and thereafter every 30 min (0-, 10-, 20-, 50-, 80-, 110-, 140-, 170-, and 200 min). Blood glucose levels were measured using a Bayer Contour hand-held glucometer (Bayer Diabetes, Mississauga, ON, Canada). Area under the glucose curve was calculated using GraphPad Prism 6 (GraphPad Software, San Diego, CA, USA) for each animal as a measure of glucose clearance [50].