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RNA extraction, cDNA conversion, primer design, and qRT-PCR was used to analyze the transcript expression patterns of the putative genes and the results were estimated using the delta-delta CT method [51,53]. qRT-PCR experiments were performed from three independent biological replicates.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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