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The SARS-CoV-2 pseudovirus was prepared by transfecting the plamids pMDL, pREV, pcDNA3.1-SARS2-Spike (Addgene #145032), and pHIV7-eGFP-ffLuc into HEK293T cells by calcium phosphate precipitation. Virus containing medium was collected 3 days after transfection. The VSV-G pseudovirus was prepared by transfecting the plamids pMDL, pREV, pVSV-G, and pHIV7-eGFP-ffLuc into HEK293T cells by calcium phosphate precipitation as we described previously (Cui et al., 2016, 2017). Virus containing medium was collected 3 days after transfection.
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This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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