2.3. DNA Extraction, PCR, Sequencing and Phylogenetic Analyses

Total genomic DNA was extracted using an M5 Fungal Genomic DNA Kit (Mei5 Biotechnology Co., Ltd., Beijing, China) and an Ezup Column Fungi Genomic DNA Purification Kit (Sangon Biotech Co., Ltd., Shanghai, China) according to the manufacturers’ instructions. For the polymerase chain reaction (PCR) amplification, primers ITS1 and ITS4 [30] were used for the ITS region while primer LR0R was paired with LR6 and LR7 in order to obtain sequences for the 28S region [31]. The reactions were performed with the following program: initial denaturation at 94 °C for 5 min (ITS) or 4 min (28S), 33 cycles at 94 °C for 30 s, 46 °C (28S) or 53 °C (ITS) for 30 s or 45 s (28S), and 72 °C for 30 s (ITS) or 40 s (28S), and for terminal elongation, the reaction batches were incubated at 72 °C for 10 min. The PCR products were examined on a 1% agarose gel detected by a JY 600 electrophoresis apparatus (Beijing JUNYI Electrophoresis Co., Ltd., Beijing, China) and then sent to BGI Co., Ltd. (Beijing, China) for sequencing.