Twenty-five Wistar rats (males and females) of 4–5 weeks of age were obtained from the Isolation and Bioterium of the Multidisciplinary Research Unit (UIM) of FES-Cuautitlan-UNAM. Rats were housed under specific conditions in an air-conditioned room and fed standard laboratory food and Milli-Q water ad libitum according to institutional guidelines from the Universidad Nacional Autonoma de Mexico. The experimental procedures were reviewed and finally approved by the Ethics Committee of FES-Iztacala-UNAM (Protocol code CE/FESI/102021/1431 and approval date: 29 October 2021).
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