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ROS was measured using the OxiSelect Intracellular ROS Assay Kit from Cell BioLabs (San Diego, CA, USA). Whole liver tissue (20 μg) was lysed using RIPA buffer for 30 min on ice before centrifugation at 12,000× g for 5 min. Samples were incubated with DCFH-DA for 60 min at 37 °C and fluorescence measured at ex/em 480/530 nm.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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