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A protease assay was performed using skim milk powder. Skim milk agar plates were prepared using 1% skim milk powder. The bacterial colonies were inoculated in broth and allowed to grow overnight. The overnight culture was streaked on pre-prepared agar plates and incubated at 37 °C again overnight. A zone of lysis was observed. A study was performed with and without the presence of ZnSO4. Different concentrations of ZnSO4 were used to study its effect on protease activity.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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