After 4 weeks of supplementation with ISPG and RT, a lead sheet of 5% body weight was tied to the tail of each mouse, then was evaluated in a columnar swimming pool maintained at 28 ± 1 °C (65 cm high, 20 cm diameter, 40 cm deep). We recorded the time from entering the water to exhaustion for each mouse as endurance performance data. The mouse being not coordinated or unable to move in the water within 7 s was the criterion [11].
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