2.3. Auxin Sensitivity Assay

Immature zygotic explants were cultured on induction medium containing different levels of 2,4-D, ranging from 0 to 9 µM, for 14 days. After transfer onto development medium, somatic embryos were counted after 9 days. The embryogenic responses of WT, max3–9, and max4 explants were also calculated as a function of the number of somatic embryos produced by each explant against the concentration of 2,4 D applied in the induction medium. For GR24 and TIS108 application to explants, 10 μL solutions with the proper concentration were directly dispensed every other day on the explants simultaneously during the 14 days of the induction process according to the treatment.