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2.9. Intracellular ROS Production Assay
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The confluent HUVEC were pretreated with ECT and incubated with 20 μM CM-H2DCFDA (#C6827, ThermoFisher, Waltham, MA, USA) and then treated with TNF-α for 6 h. The measurement of fluorescence intensity was obtained by spectrofluorometer and captured under a fluorescence microscope.
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