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At D0 or D4 of adulthood, 2.2 mL aliquots of compounds dissolved in DMSO at different concentrations were spotted onto the FUDR plates as previously described [28,29]. The plates were then placed in a sterile laminar flow hood at RT to dry. For the experiments, worms were transferred onto the compound-seeded plates directly at D0 or D4 and they were exposed to SG2 for their whole lifespan.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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