Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
2.4.1. Preparation of freeze-dried soluble and insoluble protein fraction
Ask a
question
Favorite
Protein dispersions (10 wt%) were prepared according to the method mentioned in Section 2.3. The dispersions were then centrifuged at 6000 rpm for 30 min at 25 °C. The supernatant was separately collected as soluble fraction. Both fractions were subjected to freeze-drying to prepare dried powder form of soluble and insoluble proteins for further analysis.
Copyright and License information: ©2021 This journal is The Royal Society of Chemistry
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
