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Competing Endogenous RNA Network Construction

YD Yani Dong
LL Likang Lyu
HW Haishen Wen
BS Bao Shi
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Based on the ceRNA hypothesis, we constructed mRNA-miRNA-lncRNA networks. The negatively co-expressed mRNA-miRNA pairs and lncRNA-miRNA pairs were assessed by the Spearman rank correlation coefficient (SCC), with a SCC < -0.7. The co-expressed lncRNA-mRNA pairs were assessed using the Pearson correlation coefficient (PCC) with a PCC >0.9. The significance correlations between the common miRNA sponges and the two genes were calculated using the method of hypergeometric cumulative distribution. The mRNA-miRNA-lncRNA pairs were filtered with a p-value < 0.05. The interaction networks were visualized using Cytoscape software (v3.6.0).

Copyright and License information:  ©2021 Dong, Lyu, Wen and Shi.
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