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Enzyme Linked Immunosorbent Assay (ELISA)

LB Lukas Bankamp
BP Beate Preuß
AP Ann-Christin Pecher
NB Nicola Beucke
JH Jörg Henes
RK Reinhild Klein
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Anti-topo-I antibodies were analyzed by a published in-house enzyme linked immunosorbent assay (ELISA) using a recombinant full-length topoisomerase-I (Diarect, Freiburg, Germany) (32). Positive and negative standard sera were used in each test to calculate a standard curve. Results are given as absorbance x1.000. This assay is routinely performed in our lab since several years giving reliable and reproducible results.

For the demonstration of anti-AT1R antibodies to two different ELISA-kits were used: one quantitative ELISA from CellTrend GmbH (Luckenwalde, Germany) and one competitive ELISA kit from MyBioSource (San Diego, CA, USA). They were performed according to the instructions of the companies. Results are given as U/ml (CellTrend) or ng/ml (MyBioSource).

Furthermore, sera were tested for anti-ETA1 antibodies by ELISA (CellTrend GmbH).

Copyright and License information:  ©2021 Bankamp, Preuß, Pecher, Beucke, Henes and Klein
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