Patchouli and Elsholtzia in the Secretions of E2 and P4 by Follicular Granulosa Cells After Heat Stress Treatments

By the end of the culturing process, the cell-culture medium of each group was collected for E2 and P4 detections using E2 and P4 assay kits (Shanghai Enzyme-linked Biotechnology Co., Ltd.). The cell-culture medium of each group, along with the standard blank diluent samples, was added to the ELISA Kit. All procedures were conducted according to the manufacturer's protocol. The absorbance was measured at 600 nm. A standard curve was established and the hormone content levels of each sample were calculated.