2.1. Solid state fermentation with different growing condition

To study the effect of changing fermentation media parameters on secondary metabolite profile, A. awamori was initially revived in malt extract agar for 3 days. Then, inoculation of a single colony from malt agar in 150 mL malt extract broth (malt extract 15 g L⁻¹) was performed, this seed culture was allowed to grow statically for 3 days at 30 °C. Four forms of solid media including malt extract agar, nutrient agar, tryptone soya agar and solid rice media were prepared. These four media were made as follows: malt extract agar (15 g malt extract and deionized water to 1 L), nutrient agar medium (3 g beef extract, 5 g peptone, 20 g agar and deionized water to 1 L), trypticase soya agar (15 g pancreatic digest of casein, 5 g peptic digest of soybean, 5 g NaCl, 15 g agar and deionized water to 1 L), and rice medium (100 g commercially available shelled rice were immersed in 100 mL of deionized water and kept overnight prior to autoclaving).