Yeast cultures at 5 × 105 cells/mL were grown in SCH and supplemented with or without FeCl3, FeSO4, or ZnCl2, together with increasing concentrations of P. insularum homogenate. Cells were subsequently incubated at 30 °C until control cells reached midlog (optical density [OD] = 0.4 to 0.6) and then quantified via absorbance at 590 nm using a PerkinElmer Envision plate reader.
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