Quantification of phenolic acids

NE Nancy A. ElNaker
MD Mariane Daou
MO Michael A. Ochsenkühn
SA Shady A. Amin
AY Ahmed F. Yousef
LY Lina F. Yousef
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For semi-quantification (quantification without spike of isotope labeled standard in samples to indicate matrix effects) a dilution series for the phenolic acids 2,3-Dihyrdoxybenzoic acid, 3,4-Dihydroxybenzoic acid, 4-Coumaric acid, Ferulic acid, Sinapic acid and Syringic acid (Sigma, GER) was prepared in MilliQ-H2O at 0.001, 0.01, 0.1, 1, 2, 4 µg/mL. The mixture of standard dilutions was measured on the above mentioned UHPLC-MS system and data was fitted to a linear calibration curve. Figures displaying semi-quantification were developed using R 3.6.3 (https://www.rstudio.com/products/rpackages/) and ggplot2 package (https://ggplot2.tidyverse.org/). Chemical structures depicted were drawn using Marvin 20.21.0 (https://www.chemaxon.com).

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