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Taxonomic enrichment analysis and meta-analysis
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We analysed taxonomic relative abundance differences between wild and captive populations per host species using Generalized Additive Models for Location, Scale and Shape (GAMLSS) as implemented in metamicrobiomeR::taxa.compare. Subsequently, random effects meta-analysis models were applied, using metamicrobiomeR::meta.taxa, to the pool of estimates and their standard errors to evaluate the overall effects and heterogeneity across the host species. The minimum prevalence for considering microbial taxa in the meta-analysis was set in 33% of the analysed datasets.
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