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After overexpression or silencing of KLF15 in mature adipocytes stimulated by PA for 48 h, the cell culture medium was discarded. Then, the cells were continuously stimulated with 1 ml insulin solution, which contained 100 nmol/L insulin, low glucose DMEM (1 g/L), and 10% FBS. The glucose concentration of medium was detected immediately, and determined as the glucose concentration of 0 min. The glucose concentration was then measured at 30, 60, 90, 120, 150, 180, 210, and 240 min. Finally, the glucose concentration curve was drawn.
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