The human genes associated with PC were obtained from three resources: (1) 10 hub genes related to the occurrence and development of PC and 5 potential biomarkers closely related to prognosis obtained from WGCNA; (2) Therapeutic Target Database (TTD) database [34]. The TTD database was searched using the key word "pancreatic cancer"; (3) TCGA database. RNA sequencing data of pancreatic adenocarcinoma were obtained from the TCGA database.
Data were normalized and analyzed by the edgeR package [35]. Differential expression genes (DEGs) were then screened out using | log2FC |> 1 and adjust P < 0.05. The PPI data were extracted from the Search Tool for the Retrieval of Interacting Genes (STRING, https://stringdb.org/) database [36]. The PPI data with the species limited to “Homo sapiens”, and the confidence score greater than 0.7 (high confidence) were selected for further investigation.
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