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Cells were grown to mid-log phase (OD600 0.4–0.7) in rich carbon (YPD) at 22°C. Cells were shifted to poor carbon (YPG/E) using a vacuum filter apparatus with a 0.45 µM HAWP mixed cellulose ester filter. Cells were washed three times with room temperature YPG/E and then resuspended in room temperature YPG/E by pipetting and grown in a shaking water bath at 25°C.
Copyright and License information: ©2021, Sommer et al
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