24-hour bactericidal activity assays and in vitro antimicrobial susceptibility of potential AMPs against clinical isolates of Mab

Potential AMPs from the screening assays were subjected to determine the 24-hour bactericidal activity assays (corresponding to the common time of administration) with sixteen clinical Mab isolates using the protocol as described above. To observe the early antimicrobial activities, the plates were incubated for 24 h at 37°C. The samples from each well were further diluted in 0.05% Tween 80 and inoculated on Mueller-Hinton agar. After incubation for 3 days at 37°C, the colony-forming units (CFUs) were counted and calculated for the percentage of killing using the following formula: % killing = 1 - (CFU sample/ CFU control) ×100.

For the in vitro antimicrobial susceptibility, the same protocol as described above was used. The culture plates were further incubated up to 3 days at 37°C. The MIC values were read and recorded.