The status of serum anti-H. pylori IgG antibody and serum anti-CagA IgG antibody were obtained from the NHANES III surplus documents. Antibodies to H. pylori were assessed using ELISA with Pyloristat kit (Whittaker Bioproducts, Walkersville, Maryland, USA); IgG antibodies to CagA were measured using an optimal ELISA assay using recombinant CagA for the detection of anti-CagA antibody developed by Vanderbilt University [45]. Detailed methodology followed protocols developed by Blaser et al. in 1980s [46].
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