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Inter-PF rotation angle measurement

QR Qinhui Rao
LH Long Han
YW Yue Wang
PC Pengxin Chai
YK Yin-wei Kuo
RY Renbin Yang
FH Fangheng Hu
YY Yuchen Yang
JH Jonathon Howard
KZ Kai Zhang
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The inter-PF angle is defined as the lateral rotation angle between a pair of adjacent microtubule protofilaments, as described in a previous publication36. To estimate the inter-PF angles of MTD, we fitted individual tubulin dimers built from the 16-nm MTD reconstruction into the 48-nm MTD map as rigid bodies. We calculated the inter-PF angle between each pair of tubulin dimers from adjacent protofilaments using the ‘angle_between_domains’ command from PyMOL (https://pymol.org/2/). The averaged value and standard deviation were estimated from the three measurements in three representative regions of the MTD lattice, two regions close to the edges and one region in the middle.

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