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[64Cu] labeling of LLP2A-CB-TE1A1P (LLP2A)
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LLP2A peptide was radiolabeled with [64Cu] as previously described [31]. Briefly, [64Cu]Cu-chloride was diluted with 0.1 M ammonium acetate solution (pH 5.5). LLP2A (2.5 µg; 1.61 nmol) was incubated with 74 MBq of [64Cu] at 70 °C for 30–40 min with shaking. After the incubation, the radiochemical yield and purity were determined by analytical radio-high-performance liquid chromatography (HPLC) of the crude product.
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