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A 1 mM solution of SNARF-5F 5-(and 6)-Carboxylic Acid, Acetoxymethyl Ester, Acetate (ThermoFisher #S23923) by resuspending in DMSO. A 100 μl aliquot of cell suspension was collected, washed with PBS, and resuspended in 100 μl PBS. SNARF5F solution was add to 100 μl of cell suspension at a final concentration of 10 μM and incubated at room temperature for >30 minutes. Subsequent preparation for imaging cells on an agarose pad followed procedures describe above. Image acquisition conditions are described above.
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