N-glycan fractionation

LC Léa Chuzel
SF Samantha L. Fossa
MB Madison L. Boisvert
SC Samanta Cajic
RH René Hennig
MG Mehul B. Ganatra
UR Udo Reichl
ER Erdmann Rapp
CT Christopher H. Taron
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Labelled glycans were fractionated on a Dionex Ultimate 3000 HPLC equipped with a Jasco FP-2020 Plus fluorescence detector (see details in Additional file 1). Fractions containing N-glycans of interest were dried and resuspended with MQ water for use as enzyme substrates.

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