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Specimen sampling

KM Karolina Mahlerová
PJ Pavel Jakubec
MN Martin Novák
JR Jan Růžička
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Specimens were collected in 2007, 2011 and 2017 (SM3) with pitfall traps, and killed and stored in 75% or 96% ethanol. In total, we obtained 48 individuals for further morphological examination. Only the specimens stored in 96% ethanol were used for phylogenetic analysis. As the larvae were collected in the wild and were not raised to adults, the species identification could not be confirmed morphologically. Therefore, we obtained a single adult specimen of H. tenuicornis to confirm the molecular identification of the larvae as the same species. Genetic distance was calculated using the COI and 16S genes among all taxa used for the phylogenetic analysis, and the adult specimen of H. tenuicornis was used to confirm the conspecificity of the larval H. tenuicornis using DNADIST version 3.5c implemented in BioEdit v7.0.5.339.

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