Catalase (CAT) activity

SB Samiullah Burki
ZB Zeba Gul Burki
MA Muhammad Arif Asghar
IA Imdad Ali
SZ Saba Zafar
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A mixture of 100 μL of 5.9 mM H2O2 (Sigma Aldrich, USA) and 625 μL of 50 mM potassium phosphate buffer (Sigma Aldrich, USA) (pH 5.0) was prepared for the determination of catalase activity in renal tissue. To this mixture, 25 μL of supernatant was added. The catalase present in the supernatant was disintegrated to H2O2 which will eventually cause declined in its concentration in the mixture. The level of catalase activity was monitored via the decline in absorbance at 240 nm for 1 min. The change in absorbance by 0.01 as units/min reflects the activity of 1 unit of catalase [28].

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