Dornic acidity and crematocrit analyses

Dornic acidity was determined as previously described using 4 mL of breast milk. Values accepted by the Brazilian human milk bank network comprise the range of 1-8 ^oD. The crematocrit of milk samples, used to evaluate the cream content, total fat and energy value was carried out based on standardized method¹⁸, using the following calculation: cream content: cream column (mm) x 100 ÷ total column (mm) = cream%; Fat content: (cream% - 0.59) ÷ 1.46 = fat %; Total energy content: (cream% x 66.8 + 290) = Kcal/L.