Human NP cell isolation and culture

LQ Lin Qiao
SP Shi-Yuan Peng
YZ Yu-Ping Zhou
JY Jie Yin
JX Jun-Peng Xu
BC Bo Chen
HZ Huan Zhang
CZ Chao Zhu
XY Xiao-Dong Yu
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Human NP cells were isolated as previously described (24) and cultured in a commercial NP cell medium (ScienCell Research Laboratories, Inc.) containing 15% (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 100 U/ml streptomycin/penicillin at 37˚C in a humidified atmosphere with 5% CO2.

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