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Neuroscience

Categories
+ Basic technology
+ Behavioral neuroscience
- Cellular mechanisms
Cell adhesion
Cell isolation and culture
Extracellular matrix
Intracellular signalling
Lymphatic vessel
Microglia
Mitochondria
Myelin
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Protein isolation
Receptor-ligand binding
RNA Localization
Synaptic physiology
Tissue isolation and culture
+ Development
+ Nervous system disorders
+ Neuroanatomy and circuitry
+ Peripheral nervous system
+ Sensory and motor systems
Protocols in Past Issues

Preparation and Manipulation of Olfactory Epithelium Explant Cultures for Measurement of the Mechanical Tension of Individual Axons Using the Biomembrane Force Probe
CF Coralie Fouquet
AT Alain Trembleau
Q&A 0 Q&A
6054 Views
Apr 20, 2019
In this paper, we describe a protocol allowing measurement of the mechanical tension of individual axons grown ex vivo from neural tissue explants. This protocol was developed with primary cultures of olfactory epithelium explants from embryonic (E13.5) mice. It includes a detailed description of explant dissection and culture, as well as the main steps of the procedure for axon tension measurement using the previously established Biomembrane Force Probe.