Materials and Reagents

100-1,000 µl pipette tips (KASVI, catalog number: K8-1000B)
Eppendorf Safe-Lock Tubes, 1.5 ml (Eppendorf, catalog number: 0030120086)
Amber, write-on spot, certified, 2 ml, screw top vial packs (Agilent Technologies, catalog number: 5182-0554)
Serum, colonic luminal content and feces of C57BL/6 mice
Hydrochloric acid, 37% (v/v) (Sigma-Aldrich, catalog number: 320331)
Anhydrous citric acid (Cinética Produtos Químicos, catalog number: 278)
Sodium chloride (Cinética Produtos Químicos, catalog number: 415)
Tetrahydrofuran (Merck, catalog number: 1081012500)
Acetonitrile (Sigma-Aldrich, catalog number: 60004)
N-butanol (Sigma-Aldrich, catalog number: 34867)
Acetic acid, analytical standard, GC assay ≥ 9.8% (Sigma-Aldrich, catalog number: 71251)
Propionic acid, analytical standard, GC assay ≥ 9.8% (Sigma-Aldrich, catalog number: 94425)
Butyric acid, analytical standard, GC assay ≥ 9.8% (Sigma-Aldrich, catalog number: 19215)
HCl
Distilled water
1-methylnaphthalene
N₂
H₂
0.1 M HCl solution (see Recipes)
3,000 µg/ml acetic acid in serum stock solution (see Recipes)
3,000 µg/ml propionic acid stock solution (see Recipes)
3,000 µg/ml butyric acid stock solution (see Recipes)
1,000 µg/ml SCFAs standard mixture (see Recipes)

Equipment

J&W DB-23 GC column, 60 m, 0.32 mm, 0.25 µm, 7 inch cage (Agilent Technologies, catalog number: 123-2362)
Forceps
Freezer FE26/127V (Electrolux Appliances, model: FE26, catalog number: 04251FBA106/ 04251FBB206)
Stainless steel double spatula, 180 mm length x 3 mm diameter (Metalic Acessórios para Laboratório, catalog number: 063-A3)
PIPETMAN Classic P1000 pipette (Gilson, catalog number: 20170-170)
Gas Chromatograph Agilent 6850 series (Agilent Technologies, discontinued by the manufacturer)
DB 23 Agilent capillary column, 60 m x 0.25 mm internal diameter (i.d.)
Oven
Automatic liquid sampler 7683B (Agilent Technologies, catalog number: G2880A)
Analytical balance (SHIMADZU, model: ATX224 series, catalog number not found)
Vortex mixer (Phenix Luferco, model: AP56)
Centrifuge 5810 R (Eppendorf, model: 5810 R, catalog number: 5810000424)
Gas Chromatograph Shimadzu 2010 model, equipped with FID (SHIMADZU, catalog number: C184-E019)
AOC-20i automatic liquid sampler (SHIMADZU, model: AOC-20i)

Software

EZChrom software, 3.3.1 version (Agilent Technologies)
GC solution software, 3.2 version (SHIMADZU)

Procedure

Sample preparation
1. Collect blood from cardiac puncture or axillary plexus. Maintain blood at room temperature (RT) for 30 min and centrifuge (3,000 x g, 8 min). Collect the serum and freeze at -80 °C in 1.5 ml microtubes.
2. Collect fecal pellets directly from each mouse in microtubes. For this, raise the animal holding the tail, position the microtube in the proximity of the anus and collect the pellets that are excreted. Two or three pellets from each animal are adequate.
3. After euthanasia, collect the colons and gently remove the luminal contents of the proximal part. To do this, use the forceps to collect directly into microtubes and freeze at -80 °C.
4. Keep all biological samples frozen at -80 °C until the day of analysis.
5. For feces and colonic luminal content, transfer 20 mg of these into 1.5 ml microtubes and add 200 µl of distilled water. Homogenize using a metal spatula.
6. In 1.5 ml microtubes, add the sample (200 µl serum or homogenate of feces with water, see Step A5), a 200 µl mixture of organic solvents composed of N-butanol, tetrahydrofuran and acetonitrile in a 50:30:20 ratio, 40 µl HCl 0.1 M, 20 mg citric acid and 40 mg sodium chloride. Shake the microtubes vigorously using the vortex stirrer for 1 min.
7. Centrifuge the samples at 14,870 x g at room temperature for 10 min.
8. Using an automatic pipette, transfer the supernatant to chromatographic vials equipped with 200 µl inserts and analyze by GC-FID (Figure 1).
  
  Figure 1. Sample preparation. A. Liquid-liquid extraction procedure of samples. Serum samples (200 µl) were extracted with the same volume of a mixture of solvents. B. Liquid-liquid extraction procedure for feces and colonic luminal content samples. Fecal samples (20 mg) were previously homogenized with distilled water and subjected to the extraction procedures described in Steps A5-A8 of Sample preparation section.
GC procedure
1. Adjust the temperature of the injector to 250 °C.
2. Inject contents (5 µl) in a split ratio of 25:1, using the DB 23 capillary column Agilent, 60 m x 0.25 mm of internal diameter (i.d.), and coated with a film of 0.15 µm composed of 80.2% 1-methylnaphthalene.
3. The mobile phase is composed of N₂ at an initial flow rate of 1 ml/min and maintaining this for 1 min, then changing to 0.8 ml/min for 1 min, changing to 0.6 ml/min for 1 min and then reverting to 1 ml/min for 9.2 min.
4. Adjust and maintain the temperature of the FID detector at 260 °C.
5. Adjust the flow of the H₂ and the synthetic air to 30 ml/min and 350 ml/min, respectively.
6. Adjust the temperature program of the oven to100 °C, maintain for 7 min and increase to 200 °C at a rate of 25 °C/min and maintain for 5 min.

Data analysis

Chromatograms generated by the analysis of the serum (Figure 1A) and the feces (Figure 1B) are shown in Figure 2. SCFAs are identified by the times of retention in comparison with analytical standard solutions.

Figure 2. Chromatogram of serum (A) and fecal (B) samples. The retention times of each SCFA are as follows: acetate, 5.2 min; propionate, 7.1 min; butyrate, 9.08 min. The analysis is performed using a Gas Chromatograph Shimadzu 2010 model equipped with 7683B automatic liquid sampler and FID.
Use the EZChrom software to integrate the areas of each peak, corresponding to its respective SCFA.
Prepare standard calibration curves in triplicate, with a concentration range of 15-1,000 µg/ml for SCFAs using the matrices in this study (serum and feces) as solvent to dilute the analytes. Construct the calibration curve in serum matrix, make a 1,000 µg/ml stock solution of SCFA using serum as solvent, and dilute serially in a range of 15-1,000 µg/ml (see Recipes).
Construct the calibration curve in feces matrix, make a 1000 µl/ml SCFAs using distilled water as solvent in accordance with obtaining method of stock solution of SCFA in serum (see Recipes).
Prepare samples according to the liquid-liquid extraction procedures described in the Procedure A, Steps 5-8 and analyze the samples by GC-FID as described in the Procedure B, GC procedure.
The standard calibration curves have presented linear regression relationships for all acids (R² = 0.998) (Figure 3).

Figure 3. Linearity of standard calibration curves of (A) serum and (B) feces. Calibration curves obtained using serum (A) and feces (B). Linearity obtained using Gas Chromatograph Agilent 6850 series, equipped with 7683B automatic liquid sampler and FID following the parameters described in this article.
The retention time is 7.2, 8.2, and 9.2 min for acetic, propionic and butyric acid, respectively and the total run time for each analysis is 16.5 min.
The precision, expressed as relative standard deviation (RSD) was less than 15.0%, and the accuracy, expressed as Relative Error (RE) was within ± 15.0%. The extraction efficiency (EE) was greater than 97.0%. To calculate the RSD, RE and EE, use the formulas described below:
For standard curves, plot areas of each SCFA standard sample on the y-axis against your respective theoretical concentration values (15-1,000 µg/ml) and obtain the equation of the function y = ax + b, where a is the angular coefficient, x is the area found for the sample and b is the linear coefficient.
Use the equation showed below to calculate the concentrations of each SCFA in the samples. The concentration of each SCFA is expressed as mg/ml.
Convert the concentrations for mM and mmol/kg for the serum and fecal/colonic luminal content samples, respectively using the formulas described below:

Notes

Agilent equipment is not available in our laboratory. Thus, we have used the GC Shimadzu 2010 model to obtain the chromatograms to illustrate analytes separation (Figures 2 and 4). The chromatograms were obtained following the bioanalytical method conditions described in this article.

Figure 4. Overlap of chromatograms obtained of colonic luminal content from antibiotic-treated mice and control animals (not treated with antibiotics). The chromatogram shows animals treated with antibiotics (blue line) and untreated animals (black line) (Fellows et al., 2018). The analyses are performed using a Gas Chromatograph Shimadzu 2010 model equipped with AOC-20i automatic liquid sampler and FID, in accordance with the parameters described in this article. The chromatograms were integrated using the GC solution software.
The retention time and the detection range of the analytes may vary according to capillary column type and length and equipment brands. DB-23 capillary column Agilent can be used in GC Shimadzu 2010 model.
This method was developed and validated in accordance with the RDC 27/2012 of ANVISA (National Sanitary Vigilance Agency).
No alterations to the protocol are required if different mouse strains are used.
Animals were housed in facility of the Institute of Biomedical Sciences, University of Sao Paulo and Institute of Biology, University of Campinas.
Blank serum is the serum without SCFAs standards addition.

Recipes

0.1 M HCl solution
0.835 ml HCl 37% (v/v)
Add 99.165 ml distilled water
3,000 µg/ml acetic acid in serum stock solution
5.7 µl analytical standard acetic acid, GC assay ≥ 99.8%
Add 2,000 µl blank serum
Vortex for 1 min
3,000 µg/ml propionic acid stock solution
6.06 µl analytical standard propionic acid, GC assay ≥ 99.8%
Add 2,000 µl blank serum
Vortex for 1 min
3,000 µg/ml butyric acid stock solution
6.2 µl analytical standard butyric acid, GC assay 99.8%
Add 2,000 µl blank serum
Vortex for 1 min
1,000 µg/ml SCFAs standard mixture
Mix 1 ml each SCFA stock solution to give 1,000 µg/ml each SCFA (final volume 3 ml)
Standard SCFA solutions
1. Perform a serial dilution of this mixed organic acid solution using serum as solvent to generate a range of 15-1,000 µg/ml as a standard curve of SCFAs in serum matrix
2. Perform the same serial dilution using distilled water as matrix for the construction of the standard curve of SCFAs in feces matrix. To carry this out, prepare seven tubes containing 20 mg of feces and add 200 µl of their respectively solutions obtained in the serial dilution in each tube

Acknowledgments

We are grateful to Claudete Justina Valduga and Patrícia Sartorelli for providing equipment for this study. This work was supported by FAPESP 2012/50410-8 and CNPq (The National Council for Scientific and Technological Development), project number 486037/2012-6 to C.M.F, and 2017/10653-9 to M.A.V.

Competing interests

The authors have declared that no conflict of interest exists.

Ethics

All experimental procedures were approved by the Ethics Committee on Animal Use of the Institute of Biology, University of Campinas (protocol number 3742-1), and Animal Care Committee of the University of Sao Paulo (protocol number. 181/114/02). Also, it was performed in accordance with the guidelines of the Brazilian Control and Experimentation Committee.

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