Abstract
This protocol is designed to quantify invadopodia formation and activity. Invadopodia are protrusive structures elaborated by cancer cells that mediate cell attachment and remodeling of the extracellular matrix. These structures contribute to the ability of cancer cells to invade and metastasize. In this protocol, both the presence of invadopodia and their activity is simultaneously assessed and quantified by a fluorescent microscopy-based assay.
Keywords: Cancer, Invasion, Invadopodia, Metastasis, Gelatin
Materials and Reagents
Equipment
Procedure
Acknowledgments
This protocol was initially adapted from: Mueller et al. (1992). The first adaptation was published in: Berdeaux et al. (2004). It was later implemented in: Díaz et al. (2013). Funding during first adaptation: National Institutes of Health grant CA17542 to G.S. Martin. Funding during implementation: National Institutes of Health CA129686 to S. A. Courtneidge. Other Acknowledgments: The author would like to acknowledge Dr. G.S. Martin (UC Berkeley, California) and S.A. Courtneidge (UHSU, Portland, Oregon) for their support over the time this protocol was adapted and implemented.
References
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