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Serial Transfer of Human Hematopoietic and Hepatic Stem/progenitor Cells   

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Original research article

A brief version of this protocol appeared in:
Stem Cells
Jun 2013

Abstract

A range of assays have been developed to determine the stemness or stem cell activity of human stem cells. The key assays of stem cells are functional: they must show self-renewal and the ability to generate the appropriate tissue. The best assays available to study this property in putative human stem cells involve xeno-transplantation into immune-deficient mice. Demonstration of both long-term (2-3 months) multi-lineage reconstitution of human blood or liver in a murine host and the ability of the putative stem cells to mediate reconstitution of a secondary host upon re-isolation from the primary mouse are generally accepted as the gold standard for demonstrating the presence of human hematopoietic and hepatic stem cells. Here, we describe a method of reconstituting NOD-scid IL-2Rγ-/-(NSG) mice with CD34+ stem cells from human fetal liver and repurification of CD34+ cells for serial transplantation.

Keywords: Hematopoietic progenitor, Hepatic progenitor, Immune system, Human Hepatocyte, Chimera mouse

Copyright: © 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Chen, Q. and Chen, J. (2013). Serial Transfer of Human Hematopoietic and Hepatic Stem/progenitor Cells. Bio-protocol 3(23): e992. DOI: 10.21769/BioProtoc.992.
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