Abstract
Chick embryos are known to be a powerful system to test gene function due to the “in vivo” accessibility, short time for results retrieval and the possibility to perform a large number of experiments. Synthetic micro-beads soaked in morphogenetic signals or receptor inhibitors can be implanted in selective embryo regions at precise developmental stages activating or blocking different signaling pathways. Here, we describe the manipulation of Wnt signaling pathway using Dkk-1-soaked micro-beads, implanted in ovo in the anterior part of the developing neural tube of chicken embryos; specifically at the prospective zona limitans intrathalamica at stage HH10.
Keywords: Experimental embryology, Neutral tube, Wnt signaling regulation, Microbeads implant, Ni vivo experimentos
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was adapted from the previously published studies, Crossley et al. (1996) and Vieira and Martinez, (2005), and it was performed by Martinez-Ferre et al. (2013). This work was supported by EUCOMMTOOLS Contract 261492, Spanish Ministry of Science and Innovation Grant BFU-2008-00588, Spanish Ministry of Education and Science-Universitary Professor Formation Grant AP2009-3644, Consolider Grant CSD2007-00023, Institute of Health Carlos III, Spanish Cell Therapy Network and Research Center of Mental Health, General Council of Valencia (Prometeo 2009/028 and 11/2011/042), and the Alicia Koplowith Fondation. We thank M. Ródenas for technical assistance.
References
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