Abstract
This is a protocol to detect HIV-1 reverse transcription products in cytoplasmic and nuclear fractions of cells infected with VSV-G-pseudotyped envelope-defective HIV-1. This protocol can also be extended to HIV-1 with regular envelope.
Keywords: HIV-1, Reverse Transcription, Cytoplasm, Nucleus, Quantitative PCR
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol is adapted from Shah et al (2013). This protocol was supported by NIH grant AI076121 to C.A.
References
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